Assessment of in vitro biofilm forming by Pseudomonas aeruginosa and Staphylococcus aureus isolated from bladder catheters of hospitalized patients in Medea (Algeria) and efficacy of antiseptics and antibiotics as antibacterial agents

Abstract

Both Pseudomonas aeruginosa and Staphylococcus aureus have the ability of forming biofilms and are prominent causes of medical device and implant-related infections. The purpose of this study was to assess the potential of clinical isolates of P. aeruginosa and S. aureus isolated from bladder catheters to produce biofilms. Conventional microbiological techniques were used to identify 26 and 24 clinical isolates of P. aeruginosa and S. aureus, respectively, from catheters retrieved from different services (Internal Medicine, Medical and surgical emergency, Postoperative, ENT, Traumatology, Surgery, and Maternity) of public establishment of Medea (Algeria). These strains were evaluated for biofilm development using a microtiter plate assay. According to the CRA test, 69.23% of P.aeruginosa strains and 41.66% of S.aureus strains were likely to create slime, indicating biofilm-producing bacteria. TCP method demonstrated that 30.76% of P. aeruginosa and 41.66% of S. aureus isolates had the potential to create slime. In contrast, no biofilm was developed when povidone was present. It is essential to test for biofilm formation in response to an external limitation, such as antiseptics and antibiotics, so as to create novel strategies for combating bacterial biofilms and better controlling their formation.